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KMID : 1161420170200080804
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Journal of Medicinal Food
2017 Volume.20 No. 8 p.804 ~ p.811
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Safety Assessment of Oil from Pequi (Caryocar brasiliense Camb.): Evaluation of the Potential Genotoxic and Clastogenic Effects
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Traesel Giseli Karenina
de Araujo Flavio Henrique Souza
Castro Luis Henrique Almeida
de Lima Fernando Freitas
Menegati Sara Emilia Lima Tolouei
Justi Priscilla Narciso
Kassuya Candida Aparecida Leite
Cardoso Claudia Andrea Lima
Argandona Eliana Janet Sanjinez
Oesterreich Silvia Aparecida
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Abstract
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Genotoxic data of medicinal plants and functional foods are required as part of the risk assessment by international regulatory agencies. Due to its food consumption and ethnopharmacological relevance, pequi oil (Caryocar brasiliense Camb.) is one of these compounds to be studied. The aim of this study was to evaluate the cytotoxic, genotoxic, and clastogenic effects of the oil from the pulp of C. brasiliense (OPCB) in vivo and in vitro. Initially, the Artemia salina in vitro assay was conducted to determine the cells viability rate of different doses of the OPCB. Subsequently, comet assay (Organization for Economic Cooperation and Development, OECD 489) and micronucleus test (OECD 474) were performed in blood and bone marrow of Wistar rats treated orally with a 125, 250, 500, or 1000?mg/kg/bw of the OPCB for 4 weeks. The chemical analysis indicated the presence of β-carotene and lycopene in the oil. In the A. salina test, all OPCB doses maintained cell viability rates statistically similar to the negative control. The in vivo tests performed showed that OPCB did not show significant genotoxic or clastogenic effects in cells analyzed with the four doses tested. Altogether, these results indicate that, under our experimental conditions, C. brasiliense fruit oil did not reveal genetic toxicity in rat cells.
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KEYWORD
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brazilian folk medicine, comet assay, food toxicology, functional food, genotoxicity, micronucleus, mutagenesis, rats
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